Figure 4.

WIPF1 knockdown suppressed the tumor growth of GC cells in vivo

(A) The nude mouse model and subcutaneous tumors in the sh-Ctrl group (upper panel) and sh-WIPF1 group (lower panel) at 2 and 5 weeks. The tumor volume was statistically analyzed from weeks one to five.

(B) The tumor weight was statistically analyzed and plotted in columns.

(C) IHC analysis was performed to determine the protein expression of Ki67 in tissues of the sh-Ctrl and sh-WIPF1 groups.

(D) The nude mouse model of lung metastasis was established by the tail vein injection of tumor cells in the sh-WIPF1 group.

(E) The number of tumor nodules on the lung surface was statistically analyzed in the sh-Ctrl and sh-WIPF1 groups.

(F) H&E staining of tumor nodules. ∗p < 0.01.