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. 2023 Oct 20;26(11):108273. doi: 10.1016/j.isci.2023.108273

Figure 7.

Figure 7

WIPF1 was necessary for MYOCD to promote GC cell proliferation, invasion, and migration by activating the PI3K/AKT signaling pathway

(A) The proliferative ability of the AGS and MKN45 cells infected with sh-Ctrl, sh-MYOCD, or sh-MYOCD + O-WIPF1 was determined by CCK8 assay.

(B) Detection of AGS and MKN45 cell proliferation in each group using EdU staining. The EdU-labeled cell showed red fluorescence, and DAPI staining showed blue fluorescence in the cell nucleus.

(C) The cell invasion ability of AGS and MKN45 cells infected with sh-Ctrl, sh-MYOCD, or sh-MYOCD + O-WIPF1 was evaluated by Transwell assay.

(D) A wound healing assay measured the migration of AGS and MKN45 cells at 0 and 24 h.

(E) A Western blot assay was used to verify that MYOCD activated the PI3K/AKT signaling pathway in the form of dependence on WIPF1. ∗p < 0.05, ∗∗p < 0.01.