Table 1.
Summary of single-cell sequencing technologies and platforms.
| Technology | Platform | Year | Single-cell Isolation Method | Barcode Type | Library Preparation Method | Throughput |
|---|---|---|---|---|---|---|
| Transcriptomics | SMART-seq2 | 2013 | FACS | Unique Molecular Identifier | Full-length cDNA amplification | Low |
| Drop-seq | 2015 | Droplet-based microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| inDrop | 2015 | Droplet-based microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| 10x Genomics Chromium | 2015 | Microfluidics, FACS | Unique Molecular Identifier | cDNA amplification | High | |
| CytoSeq | 2015 | microwells | Unique Molecular Identifier | cDNA amplification | High | |
| Seq-Well | 2017 | Microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| MARS-seq | 2017 | Microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| sci-RNA-seq2 | 2019 | Microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| CEL-Seq2 | 2015 | Micromanipulation, FACS | Unique Molecular Identifier | cDNA amplification | Low | |
| Quartz-Seq | 2017 | Microfluidics | Unique Molecular Identifier | cDNA amplification | High | |
| SPLiT-seq | 2017 | Micromanipulation, FACS | Unique Molecular Identifier | cDNA amplification | High | |
| Genomics | 10x Genomics Chromium | 2015 | Microfluidics, FACS | Unique Molecular Identifier | Genome amplification | High |
| MARS-seq | 2017 | Microfluidics | Unique Molecular Identifier | Genome amplification | High | |
| Seq-Well | 2017 | Microfluidics | Unique Molecular Identifier | Genome amplification | High | |
| sci-RNA-seq2 | 2019 | Microfluidics | Unique Molecular Identifier | Genome amplification | High | |
| CEL-Seq2 | 2015 | Micromanipulation, FACS | Unique Molecular Identifier | Genome amplification | Low | |
| SPLiT-seq | 2017 | Micromanipulation, FACS | Unique Molecular Identifier | Genome amplification | High | |
| Epigenomics | scATAC-seq | 2015 | Tn5 tagmentation | Unique Molecular Identifier or ATAC-barcode | Genome amplification/tagmentation | High |
| scCHIC-seq | 2017 | Tn5 tagmentation | Unique Molecular Identifier or ATAC-barcode | Genome amplification/tagmentation | High | |
| scNMT-seq | 2017 | Tn5 tagmentation | Unique Molecular Identifier | Genome amplification | High | |
| scCOOL-seq | 2019 | Tn5 tagmentation | Unique Molecular Identifier | Genome amplification | High | |
| scTHS-seq | 2020 | Tn5 tagmentation | Unique Molecular Identifier | Genome amplification | High | |
| scMC-seq | 2020 | Tn5 tagmentation | Unique Molecular Identifier | Genome amplification | High | |
| Spatial Transcriptomics | 10x Genomics Visium | 2016 | Spatial capture, in situ sequencing | Spatial barcode | cDNA amplification | High |
| Slide-seq | 2020 | Spatial capture, in situ sequencing | Spatial barcode | cDNA amplification | High | |
| MERFISH | 2016 | Single-molecule FISH | Oligonucleotide probes | Imaging | ||
| seqFISH+ | 2017 | Single-molecule FISH | Oligonucleotide probes | Imaging | ||
| osmFISH | 2019 | Single-molecule FISH | Oligonucleotide probes | Imaging | ||
| STARmap | 2019 | Single-molecule FISH | Oligonucleotide probes | Imaging | ||
| FISSEQ | 2019 | Single-molecule FISH | Oligonucleotide probes | Imaging |