Fig. 3. JHU083 lacks therapeutic efficacy in Mtb-infected SCID mice.

a Schematic of the SCID mouse experiments. Six to ten weeks old Balb/c SCID mice (n = 5–10/group) were aerosol infected with ~40 CFU of Mtb H37Rv. The mice were then treated with JHU083 or rifampin (RIF) by oral gavage starting one day after infection. 1 mg/kg JHU083 was given daily for the first 5 days and then the dose was reduced to 0.3 mg/kg daily (5/7, M-F). b Lung CFU burden in SCID mice (n = 5/group) treated with JHU083, RIF, or PBS. Mice were sacrificed at week 5 post-infection/treatment. The lungs were harvested, homogenized, diluted, and plated on 7H11 selection plates. After 21–25 days, the colonies were counted, and counts were transformed into log₁₀ values and plotted. c Body weights of SCID mice treated with JHU083, RIF, or PBS (n = 10/group). d Survival of SCID mice treated with JHU083, RIF, or PBS (n = 10/group). Data were plotted as mean ± SEM. Statistical significance was calculated using a two-tailed student t test considering unequal distribution. For survival curve, log-rank (Mantel-Cox) and Gehan-Breslow-Wilcoxon tests were used and yielded similar p-value. The exact p-values are provided in the Source Data file. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. CFU stands for colony-forming units. NS stands for non-significant change, p-value was >0.05. The experiment was repeated twice. The mouse clipart shown in 3a was created using Biorender.com (Tornonto, Canada) by SP.