Fig. 5. JHU083 treatment modulates lung myeloid cell populations.

As described in Fig. 2a, Mtb-infected 129S2 mice were treated with JHU083, RIF, or PBS every day starting at day 1 post-infection (n = 5/group). The mice were sacrificed on week 2 and week 5, and the lungs were harvested. Single cell suspension of the lungs from all three treatment groups were stained with appropriate antibodies and analyzed using multicolor-flow cytometry (n = 5 mice). Details are provided in “Methods” section. We found differences in the frequencies or gMFI values for (a) CD11b⁺ myeloid cells, (b) alveolar macrophages (AM, CD11b⁺ SiglecF⁺), (c) CD86 expression upon AM, (d) CD206 expression upon AM, (e) interstitial macrophages (IM, CD11b⁺ SiglecF^- F4/80⁺), (f) IL-10 expression upon monocytic myeloid-derived suppressor cells (mMDSCs, CD11b⁺ Ly6G^- Ly6C^High), (g) IL-10 expression upon granulocytic myeloid-derived suppressor cells (gMDSCs, CD11b⁺ Ly6G⁺ Ly6C^low). The X-axis indicates the lung harvest timepoint. Data were plotted as mean ± SEM and are shown as the frequency of CD45⁺ population. gMFI was mostly used for low abundance cell surface markers and transcription factors. Statistical significance was calculated using a two-tailed student t-test considering unequal distribution. The exact p-values are provided in the Source Data file. * < 0.05, ** < 0.01. NS stands for non-significant change, p-value was >0.05. The experiment was repeated twice.