Fig. 4. Astrocyte specificity of 4x6T cassette is preserved for long time periods and across CNS regions.

a Astrocyte specificity 6 months after injection (retroorbital injection, 5 × 10¹¹ vg/mouse PHP.eB::GfaABC1D-Cre-4x6T; 5 × 10¹¹ vg/mouse PHP.eB::CAG-flex-lck-smV5-4x6T) into young adult (2–5-month-old) mice is preserved in the cortex (scale bar: 40 μm) and to a slightly lesser degree in the hippocampus. Hippocampus, left: entire structure (scale bar: 150 μm); yellow box shows the region of higher magnification on the right (scale bar: 20 μm). Yellow arrow: example of neuron in the dentate gyrus. Astrocyte specificity is higher in the cortex than the dentate gyrus sub-region (*P = 0.0134); Kruskal–Wallis test, Dunn’s multiple comparisons test, P = 0.0024, Kruskal–Wallis statistic 8.346). Mean ± SEM; n = 4 mice per brain region. Source data are provided as a Source Data file. b Sagittal section of mouse brain 6 months after virus injection (scale bar: 1 cm), with higher magnification examples of the cerebellum, thalamus, and olfactory bulb (scale bars: 100 μm), showing high levels of colocalization of V5 with Sox9. c Coronal section of mouse spinal cord 6 months after virus injection (scale bar: 100 μm); yellow box denotes area of higher magnification section on the right (scale bar: 20 μm), showing high V5/Sox9 colocalization; similar observations in four mice in each CNS region.