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. 2023 Nov 3;11:1291824. doi: 10.3389/fbioe.2023.1291824

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Copyright © 2023 Li, Li, Li, Wang, Tan and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative immunofluorescence staining of FAP protein in U87MG cell. FAP was indicated by positive staining (green), and nuclear condensation was indicated by DAPI nuclear staining (blue). Scale bar: 100 μm; (B) Viability of U87MG cells incubated with the different radioactive concentrations of [⁶⁸Ga]Ga-FAP-2286-ICG for 24 h (n = 3); (C) The radioactivity uptake of [⁶⁸Ga]Ga-FAP-2286-ICG in U87MG cells; (D) Cellular uptake of [⁶⁸Ga]Ga-FAP-2286-ICG compared to [⁶⁸Ga]Ga-FAP-2286 in U87MG cells; Representative optical image (E) and quantification (F) of U87MG cells interaction after incubation for 2, 4, 8, and 24 h with 3 μM of either FAP-2286-ICG or ICG (n = 5). ns: not statistically significant; *p < 0.05; **p < 0.01.