TABLE 4.

Conversion rates of C_12:0 to C_18:0 fatty acids to ω-1, ω-2, and ω-3 hydroxy fatty acid products by E. coli K27(pCYP102, pGEc47)^a

Substrate fatty acid	Hydroxy fatty acid product formation (U g−1 cdw)			Total product formation (Ug−1 cdw)
	ω-1b	ω-2b	ω-3b
C12:0	0.25 ± 0.04	≤0.17	0.21 ± 0.03	0.63 ± 0.07
C13:0	≤0.08	0.63 ± 0.07	≤0.13	0.84 ± 0.07
C14:0	0.83 ± 0.1	0.42 ± 0.05	0.42 ± 0.05	1.67 ± 0.2
C15:0	0.42 ± 0.05	0.63 ± 0.07	0.31 ± 0.04	1.36 ± 0.12
C16:0	≤0.13	≤0.19	≤0.10	≤0.42
C17:0	≤0.07	≤0.08	≤0.05	≤0.20
C18:0	≤0.03	≤0.04	≤0.03	≤0.10

^aRates of product formation calculated by GC analysis are based on a 4-h fatty acid conversion and three determinations. 

^bPosition of the hydroxy group in the hydroxy fatty acid.