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. 2023 Nov 13;39(13-15):817–828. doi: 10.1089/ars.2023.0303

FIG. 1.

FIG. 1.

Temporal relationship between duration of high glucose exposure and expression of LncCytB and CYTB. HRECs incubated in high glucose for 12, 24, 48, 72, and 96 h were analyzed for (a) LncCytB transcripts by strand-specific PCR, and (b) CYTB mRNA by qRT-PCR using β-actin as a housekeeping gene. (c) Effect of LncCytB regulation on CYTB mRNA. (d) Transfection efficiency of LncCytB overexpression or si-RNA, quantified by its transcripts. Each measurement was performed in triplicate in five different cell preparations, and the values are represented as mean ± SD. LncCyt, LncCytB overexpressing plasmids; LncCyt-si, LncCytB-siRNA; NG and HG = 5 and 20 mM d-glucose; HG/LnCyt, HG/LnCyt-si, HG/EV, and HG/SC, cells transfected with LncCytB overexpressing plasmids, or LncCytB-siRNA, or empty vector or with scrambled control RNA, respectively, and incubated in high glucose for 96 h; L-Gl = 20 mM l-glucose; *p < 0.05 versus NG and #p < 0.05 versus HG. EV, empty vector; HG, high glucose; HREC, human retinal endothelial cell; NG, normal glucose; qRT-PCR, real-time quantitative polymerase chain reaction; SC, scrambled control RNA; SD, standard deviation.