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. 2023 Nov 13;39(13-15):817–828. doi: 10.1089/ars.2023.0303

FIG. 3.

FIG. 3.

Regulation of LncCytB, complex III activity, and mitochondrial respiration. Isolated mitochondria were used to measure (a) complex III activity by quantifying the reduced cytochrome c at 550 nm, and (b) ROS fluorometrically using DCFH-DA. (c) OCR, (d) basal respiration, and (e) maximal respiration rates, and (f) spare respiratory capacity were measured in HRECs by Seahorse XF analyzer using Seahorse XF Cell Mito Stress Test Kit. Each sample was measured in 8–10 wells per group. Values in the graphs are mean ± SD, obtained from three different cell preparations. LncCyt, LncCytB overexpressing plasmids; LncCyt-si, LncCytB-siRNA; NG and HG = 5 and 20 mM d-glucose; HG/LnCyt and HG/EV, cells transfected with LncCytB overexpressing plasmids, or with an empty vector and incubated in high glucose for 96 h; HG/LnCyt-si and HG/SC, cells transfected with LncCytB-siRNA or with scrambled control RNA, and incubated in high glucose for 96 h; L-Gl = 20 mM l-glucose. *p < 0.05 versus NG and #p < 0.05 versus HG. DCFH-DA, 2′-7′dichlorofluorescein diacetate.