FIG. 7.

Effect of Sirt1 overexpression on LncCytB and LncCytB-CYTB interactions. Retinal microvasculature from Sirt1 overexpressing mice was analyzed for (a) LncCytB at CYTB interaction by ChIRP technique, (b) CYTB mRNA by qRT-PCR using 18S rRNA as a housekeeping gene, and (c) LncCytB transcripts by strand-specific PCR. (d) Complex III activity was quantified spectrophotometrically in retinal mitochondria by measuring reduced cytochrome c. Each measurement was performed in five or more mice per group, and the values are represented as mean ± SD. *p < 0.05 versus WT-N and ^#p < 0.05 versus WT-D. Sir-N and Sir-D, Sirt1 overexpressing normal and diabetic mice, respectively; Sirt1, sirtuin 1.