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. 2023 Nov 3;10:1275160. doi: 10.3389/fnut.2023.1275160

Figure 2.

Figure 2

Adipocyte hypertrophy, inflammation, and lipid composition in WAT depots in response to 12 weeks of HSD and HFD feeding. (A) Representative hematoxylin and eosin (H&E) sections of inguinal (iWAT, top) and gonadal white adipose tissue (gWAT, bottom) from HSD- and HFD-fed mice compared to controls. (B) Adipocyte sizes determined in iWAT and gWAT H&E stainings. (C,D) Relative abundances of triglycerides (TG) in iWAT and gWAT of HFD- and HSD-fed and control mice at the end of the study. (E–H) The expression of inflammatory genes Il1b, Mcp1 and Tnfa in iWAT (E) and gWAT (F), as well as expression of Ucp1 (G) and Fasn (H) in both iWAT (left) and gWAT (right). (I) Western blot analysis of HSL phosphorylation (pHSL (Ser660) and HSL) as well as FAS expression in gWAT (n = 4/group). (J,K) Densitometric analyses of HSL-phosphorylation (J) and FAS expression (K), both normalized for bActin expression. Color coding for all graphs: black - control diet; blue - HSD, red - HFD. Data are presented as mean ± SEM, with N = 6–8 mice per group. Statistical significance was tested by two-way ANOVA with Tukey’s (C–F) multiple comparisons test or uncorrected Fischer’s LSD (G,H), or by ANOVA with Tukey’s (B,J,K) multiple comparisons test and is indicated as follows: *, vs. REF; § HSD vs. HFD; *, § p < 0.05; **, §§ p < 0.01; ***, §§§ p < 0.001.