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. 2023 Nov 3;14:1264447. doi: 10.3389/fimmu.2023.1264447

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Copyright © 2023 Wu, Wang, Liang, Chen, Sun, Wei, Zhong, Huang, Liu, Wang, Yu and Lan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tow-color immunofluorescence detects that overexpression of SARS-CoV-2 N protein triggers Mincle-expressing macrophages infiltrating the AKI kidney at the age of 16-week db/db mice, which is inhibited by quercetin treatment. (A) Two-color immunofluorescence shows the co-localization of Mincle (green) and F4/80(red) macrophages infiltrating the kidney of db/db mice treatment with or without quercetin. (B) Semi-quantitative analysis of Mincle-expressing macrophages (F4/80⁺Mincle⁺). Each dot represents one mouse and data are expressed as the mean ± SD for groups of 6 mice. **** p<0.0001 versus empty vector control group (db/db-EV); #### p<0.0001 versus DMSO-treated control group(db/db-NP+DMSO). Scale bar=50μm.