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. Author manuscript; available in PMC: 2024 Jun 16.
Published in final edited form as: J Vis Exp. 2023 Jun 16;(196):10.3791/65301. doi: 10.3791/65301

Figure 4: Noninvasive burn wound imaging and assessment.

Figure 4:

(A) Laser speckle imaging (LSI) with proper orientation of the laser beam indicator to the center of the wound is shown in the left-side image; the right-side image shows the LSI device and the real-time skin vascular perfusion map. (B) Transepidermal water loss (TEWL) probe application to the wound site at five different spots (four wound corners and the center demonstrated in lower-right corner image) is shown in the left-side image; the right-side image is a representative real-time captured screen of the TEWL measurement. (C) Harmonic ultrasound scanning of the burn wound using a high-resolution 16 MHz ultrasound probe is shown on the left side; the right-side image shows the ultrasound device and the real-time screen recording. (D) Structural (B-mode images, grayscale ultrasound) and biomechanical (elastography, color ultrasound) images of the burn wound site at the inoculation day and day 7 post-inoculation. The wound depth is indicated by the yellow dashed line.