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. 2023 Oct 31;145(45):24535–24548. doi: 10.1021/jacs.3c05365

Figure 3.

Figure 3

Structure–activity relationship of TNB-R10-ILFF. Confocal images of HeLa cells treated with I (5 μM) and with analogues of 2 having [A(i)] different thiol-reactive moieties and [A(ii)] its corresponding quantification. Graph shows individual nuclear fluorescence intensities of 600 cells across six biological replicates. (B) Different CPP sequences and (C) different distances between the R10 and hydrophobic ILFF motif in serum-free FluoroBrite DMEM for 1 h. Scale bars, 20 μm. Graphs show individual nuclear fluorescence intensities of 300 cells across three biological replicates following cotreatment with I and CPP-additive. Red bars on the graphs correspond to the median fluorescence intensity of each treatment. P-values were determined by one-way analysis (ANOVA) followed by Tukey’s post hoc analysis. All uptake experiments were performed at 37 °C.