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. 2023 Oct 12;145(45):24785–24795. doi: 10.1021/jacs.3c08843

Figure 3.

Figure 3

Further characterization and mechanistic elucidation of ATNC: (A) WB analysis of live HeLa cells coexpressing EGFP and GBP-mCherry-LC3B was performed and the cell lysate samples were collected 1, 2, 3, 4, and 5 days after transfection; a control cell lysate sample only expressing EGFP was collected at the 5th day after transfection. (B) Statistical quantification of the WB analysis (n = 4 experiments). (C) Gradient concentration of Rap (0, 5, 10, 20, 50, and 100 nM) was added to live HeLa cells coexpressing EGFP and GBP-mCherry-LC3B, and imaging was performed 22 h after drug addition (control: cells coexpressing EGFP and mCherry-LC3B). (D) WB analysis (46 h) of the cell samples corresponding to (C). (E) Quantification of WB results (n = 3 experiments). (F) Live HeLa cells coexpressing EGFP and mCherry-LC3B (control) or GBP-mCherry-LC3B with or without adding CQ (46 h) were subjected to WB analysis. (G) Statistical quantification of WB results (n = 3 experiments). One-sided Student’s t-test was used in this figure; n.s.: nonsignificant; see Methods section for description of box plots; bar graphs denote mean ± SD; see Methods section for more details about drug treatment; mChe: mCherry.