Figure 4.
Impact of ionizable lipids and their pKa on the frequency of indel% (normalized to wild-type (WT) cells) in (A) primary human KCs and (B) bronchial epithelial cells as well as nonepithelial cells including endothelial cells and dermal fibroblasts. (C) Cell viability of primary human KCs after a 48h treatment with the different mRNA-loaded LNP formulations. Data are presented as mean ± SD of at least three biologically independent replicates. * indicates statistical significance over RNAimax: *p < 0.05; **p < 0.01; ***p < 0.001.