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Kinetic parameters of 2eGDHt with glycerol and 1,2-PDO
| Substrate | Km (mM) | Vmax (nkat mg−1) | Kcat (s−1) | Kcat/Km (s−1 mM−1) |
|---|---|---|---|---|
| Glycerol | 3.42 | 58.15 | 181.72 | 53.17 |
| 1,2-PDO | 3.70 | 106.65 | 172.29 | 46.48 |
Enzyme assay was performed by incubated with each of the substrates ranging from 2 to 200 mM at 37 °C and pH 7.0 for 10 min
