FIG. 5.

SDS-PAGE of cytoplasm and membranes of H. halophilus. Cells were grown in NB plus 0.05 M MgSO₄ (pH 7.8) containing 0.5 M NaNO₃ (lanes 2 and 7), or 0.25 (lanes 3 and 8), 0.5 (lanes 4 and 9), 1.0 (lanes 5 and 10), or 2.0 (lanes 6 and 11) M NaCl. Cultures were grown for at least two transfers in the medium indicated. Standards are shown in lanes 1 and 12. Chloride-induced proteins and their apparent molecular masses are indicated by arrows.