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. Author manuscript; available in PMC: 2024 Jan 1.

Published in final edited form as: Methods Mol Biol. 2024;2738:215–228. doi: 10.1007/978-1-0716-3549-0_14

Table 1.

Cryo-EM data collection parameters for Krios equipped with Gatan K3

Microscope Imaging Mode
Imaging Mode	Nanoprobe EFTEM
Camera Mode	Super Resolution
Cs	2.7 mm
Camera type	Gatan K3
Physical Pixel Size	5 μm
Imaging Parameters
Magnification	81,000x
Nominal Dose	50.0 e⁻/Å²
Illuminated Area	1.15 μm
Binning	0.5
Number of Frames	40
Defocus Range	−0.5 to −1.5 μm
Focus Interval	12 μm
Filter Slit	20 eV
Image Pixel Size	0.561 Å
Spot Size	7
Dose rate	13.13 e⁻/s/phys. pixel
Exposure Time	4.8 sec
Settling Time	2 sec
Defocus Step	0.25 μm
Program	Latitude
Acquisition Method	Single Particle