Skip to main content
. Author manuscript; available in PMC: 2024 Apr 27.
Published in final edited form as: Methods Enzymol. 2023 Apr 27;689:277–301. doi: 10.1016/bs.mie.2023.04.012

Fig. 4.

Fig. 4

Titration of the fluorescence emission spectra of AKR1D1 with increasing [NADPH]. (Panel A) Emission spectrum of recombinant AKR1D1 excited at 295 nm following the addition of increasing concentrations of NADPH from 0 to 4 μM. A decrease in emission is seen at 340 nm and an increase in emission is seen at 460 nm with an isosbestic point at 405 nm. (Panel B) Plot of ΔF/ΔFmax versus [NADPH], wild type (■). This research was originally published in the Journal of Biological Chemistry. Drury et al., (2010).