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. 2023 Aug 21;12(9):bio059832. doi: 10.1242/bio.059832

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Visualization of the biphasic nature of the calcium wave during fertilization. (A) Time-lapse images of the calcium wave during fertilization. Time 0 was taken as the first frame showing a visible increase in fluorescence in the oocyte. Scale bar: 100 µm. (B) Quantitation of the change in fluorescence [(F₁-F₀)/F₀)] over a region of interest encompassing the path of the oocyte during ovulation for a representative fertilization event shown above. The x-axis is time in seconds. The average intensity was plotted. Standard deviation is shown. Signals were normalized to the 15th frame before fertilization. n=7.