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. 2023 Aug 21;12(9):bio059832. doi: 10.1242/bio.059832

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — CaFE fluorescence is dependent upon functional sperm. Time-lapse images of ovulation in the EAG26 [CaFE;spe-9(hc88)] strain at the permissive temperature of 20°C (left column), the restrictive temperature of 25°C (middle column) or the control EAG16 (CaFE) strain at 25°C (right column). Scale bars: 10 μm Note that typical calcium waves associated with fertilization were observed at the permissive temperature in EAG26 and at the restrictive temperature for the control EAG16 strain.