Fig. 2.

Conditional SERT knockout in pyramidal neurons (SERT^PyramidΔ). (A) Representative images of in situ hybridization of SERT mRNA on coronal brain sections from SERT^PyramidΔ mice and SERT^fl/fl littermates at P3. White squares indicate the CA3 area shown in corresponding adjacent images at higher magnification. SERT^PyramidΔ abolished SERT expression in CA3 and mPFC, but not in the thalamus and raphe nuclei. n=4 mice/genotype. (B) Representative images of 5-HT immunostaining of coronal brain sections of SERT^PyramidΔ and SERT^fl/fl littermate mice age P6. Insets show 5-HT immunostaining and DAPI overlay at a higher magnification. In SERT^PyramidΔ mice, 5-HT immunoreactivity in CA3 and mPFC, but not in thalamic and raphe, neurons was dramatically diminished. Images are representative of three independent experiments, each with two mice/genotype. DLG, dorsal geniculate nucleus; PrL, prelimbic cortex; IL, infralimbic cortex; fmi, forceps minor; Aq, aqueduct.