Fig. 7. ZI DA axonal terminals in the PVT were activated for food approach but inhibited during food consumption.

(A) AAV-hSynapsin1-FLEx-axon-GCaMP6s was injected into ZI of TH-Cre mice for fiber-photometry imaging of ZI DA axon terminals in PVT. (B) Representative images show GCaMP6s was expressed in both ZI DA somas (top) and axonal terminals in the PVT (bottom). (C) Heatmap of normalized z-scores of ZI-PVT DA axonal terminals from 132 feeding trials of six ZI^TH-GCaMP mice. (D) z-scores of ZI-PVT DA axonal terminals aligned to the time of pellet retrieval from 132 trials of six ZI^TH-GCaMP mice. (E) Bar graph with data plots showing the averages of z-score at the time of −5, 0, and 5 s (n = 6 mice). One-way RM ANOVA with post hoc Bonferroni test.