| Reagent | Volume |
|---|---|
| Forward Oligo (100 pm) | 1 μL |
| Reverse Oligo (100 pm) | 1 μL |
| NEB ligase buffer 10× | 1 μL |
| T4 polynucleotide kinase 10 U/μL (NEB) | 0.5 μL |
| Nuclease free water | 6.5 μL |
| Total | 10 μL |
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