| Reagent | Volume |
|---|---|
| Forward Primer (10 pm) | 1 μL |
| Reverse Primer (10 pm) | 1 μL |
| Processed inoculum | 2 μL |
| Master mix 2× (Promega) | 10 μL |
| Nuclease free water | 6 μL |
| Total | 20 μL |
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