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. 2023 Nov 3;4(4):102668. doi: 10.1016/j.xpro.2023.102668
Steps Temperature Time Cycles
Initial Denaturation 95°C 10 min 1
Denaturation 95°C 20 s 35 cycles
Annealing 55°C 20 s
Extension 72°C 45 s
Final extension 72°C 10 min 1
Hold 16°C Forever