| Reagent | Amount |
|---|---|
| PEG-8000 (sigma) | 200 g |
| NaCl | 35 g |
| Milli Q water | 200 mL |
| 10x PBS (pH 7.4) HyClone | 50 mL |
| Dissolve by placing on a magnetic stirrer, with low heat if needed | |
| Makeup with MilliQ water | 500 mL |
| Vacuum filter using 500 mL 0.2 μ filter (Thermo) and store in 4°C | |
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