REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC anti-mouse CD4 Antibody	Biolegend	Cat# 100405; RRID: AB_312690
APC/Cyanine7 anti-mouse CD19 Antibody	Biolegend	Cat# 152412; RRID: AB_2922473
PE anti-mouse Ly-6C Antibody	Biolegend	Cat# 128007; RRID: AB_1186133
Alexa Fluor® 700 anti-mouse CD45 Antibody	Biolegend	Cat# 103128; RRID: AB_493715
APC/Cyanine7 anti-mouse CD11c Antibody	Biolegend	Cat# 117324; RRID: AB_830649
APC/Cyanine7 anti-mouse CD3ε Antibody	Biolegend	Cat# 100330; RRID: AB_1877170
APC/Cyanine7 anti-mouse CD11b Antibody	Biolegend	Cat# 101226; RRID: AB_830642
PE/Cyanine7 anti-mouse CD11b Antibody	Biolegend	Cat# 101216; RRID: AB_312799
FITC anti-mouse CD11b Antibody	Biolegend	Cat# 101205; RRID: AB_312788
APC/Cyanine7 anti-mouse CD45R/B220 Antibody	Biolegend	Cat# 103224; RRID: AB_313007
APC/Cyanine7 anti-mouse TER-119 Antibody	Biolegend	Cat# 116223; RRID: AB_2137788
APC/Cyanine7 anti-mouse FcεRIα Antibody	Biolegend	Cat# 134326; RRID: AB_2572064
APC/Cyanine7 anti-mouse CD5 Antibody	Biolegend	Cat# 100650; RRID: AB_2876396
APC/Cyanine7 anti-mouse Ly-6G Antibody	Biolegend	Cat# 127624; RRID: AB_10640819
FITC anti-mouse Ly-6G Antibody	Biolegend	Cat# 127605; RRID: AB_1236488
APC/Cyanine7 anti-mouse CD16/32 Antibody	Biolegend	Cat# 101328; RRID: AB_2104158
Brilliant Violet 421™ anti-mouse F4/80 Antibody	Biolegend	Cat# 123131; RRID: AB_10901171
Pacific Blue™ anti-mouse FOXP3 Antibody	Biolegend	Cat# 126410; RRID: AB_2105047
BV421 Mouse Anti-GATA3	BD Biosciences	Cat# 563349; RRID: AB_2738152
ROR gamma (t) Antibody (AFKJS-9) PE	Thermo Fisher Scientific	Cat# 12-6988-82; RRID: AB_1834470
IL-17A Antibody (eBio17B7) PE-eFluor™ 610	Thermo Fisher Scientific	Cat# 61-7177-82; RRID: AB_2574656
IL-22 Antibody (IL22JOP) APC	Thermo Fisher Scientific	Cat# 17-7222-82; RRID: AB_10597583
T-bet Antibody (eBio4B10 (4B10)) PE	Thermo Fisher Scientific	Cat# 12-5825-82; RRID: AB_925761
IFN gamma Antibody (XMG1.2) PE-Cyanine7	Thermo Fisher Scientific	Cat# 25-7311-82; RRID: AB_469680
Bacterial and virus strains
Lactobacillus acidophilus CGMCC 0460.2	Grand Pharmaceutical Group Limited	N/A
Biological samples
Mice fecal samples	This study	N/A
Mice colon tissue	This study	N/A
Chemicals, peptides, and recombinant proteins
Recombinant Murine IL-2	PeproTech	Cat# 212-12
Recombinant Murine IL-7	PeproTech	Cat# 217-17
Recombinant Mouse IL-23	Biolegend	Cat# 589002
RPMI 1640	Gbico	Cat# 11875119
IMDM	Gbico	Cat# 12440053
Fetal Bovine Serum (FBS)	Gbico	Cat# 10099141C
Penicillin/streptomycin	Gbico	Cat# 15070063
HEPES	Gbico	Cat# 15630130
EDTA	Invitrogen	Cat# AM9260G
Deoxyribonuclease I	Sigma	Cat# DN25-1G
Collagenase Type VIII	Sigma	Cat# C2139-5G
Percoll	Sigma	Cat# GE17-0891-09
Indole-3-lactic acid	Sigma	Cat# I5508
CH-223191	Sigma	Cat# C8124
Critical commercial assays
Mouse IL-1β ELISA Kit	Lianke Biotechnology	Cat# EK201B
Mouse TNF-a ELISA Kit	Lianke Biotechnology	Cat# EK282
Mouse IL-22 ELISA Kit	Lianke Biotechnology	Cat# EK222
Deposited data
16S rRNA gene sequencing data	This paper	SRA: PRJNA958442
RNA-seq dataset	This paper	GEO: GSE231473
Experimental models: Organisms/strains
ICR Mouse	SPF(Beijing) Biotechnology Co., Ltd	N/A
Oligonucleotides
Primers for quantitative PCR, see Table S1	This paper	N/A
Universal 16S rRNA V3-V4 region primers	Jin et al.55	N/A
Software and algorithms
GraphPad Prism 8.0.2	GraphPad Software	N/A
FlowJo 10 software	FlowJo LLC	N/A
Gallios flow cytometer	Beckman Coulter	N/A
MoFlo Astrios EQ	Beckman Coulter	N/A