Figure 1.

p115 is a putative downstream target of JAK/STAT signaling in Drosophila testis

(A) Dam-ID analysis for Stat92E reveals binding peaks of Stat92 E at the p115 region (orange dashed boxes).

(B) qRT-PCR quantification of p115 mRNA levels in testes with indicated genotypes. Mean ± SD is shown (n ≥ 4). Two-tailed Student’s t test was used. ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.

(C) Western blot analysis of p115 protein levels in the adult testes with indicated genotypes. β-Tubulin was used as blotting control.

(D) Quantification of p115 protein levels in testes with indicated genotypes by western blot analysis. Blot band intensity ratio of p115/β-tubulin shows similar trend with qRT-PCR in (B). Data are shown as mean ± SD (n = 3). Ordinary one-way ANOVA test was used. ^∗p < 0.05, ^∗∗p < 0.01.