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. 2023 Sep 7;18(10):1940–1953. doi: 10.1016/j.stemcr.2023.08.007

Figure 5.

Figure 5

The levels of pStat92E and Stat92E are diminished in p115-depleted GSCs

(A) The levels of pStat92E (red) and Stat92E (green, by Stat92E-Flag-GFP) in control GSCs. GSCs are indicated by white dotted circles. The hub is indicated by asterisks. pStat92E and Stat92E channels are shown separately.

(B) The levels of pStat92E (red) and Stat92E (green, by Stat92E-Flag-GFP) are greatly diminished in nos > p115RNAi GSCs.

(C) Quantification of the average fluorescent intensity of pStat92E and Stat92E in control and nos > p115RNAi GSCs. Mean ± SD is shown (n = 20). Two-tailed Student’s t test was used. ∗∗∗∗p < 0.0001.

(D) Western blot analysis and quantification of the levels of pStat92E protein in adult testes with indicated genotypes. β-Tubulin was used as blotting control. Data are shown as mean ± SD (n = 3). Two-tailed Student’s t test was used. ∗p < 0.05.

(E–I) Representative confocal images of pStat92E (yellow) staining in adult testes of control and nos > p115RNAi at different time points after eclosion. The levels of pStat92E gradually reduced over time in p115-depleted GSCs (white arrowheads). The GBs with high pStat92E levels are indicated by yellow arrowheads. The hub is indicated by yellow asterisks. pStat92E channels are shown separately.

(J) Quantification of the average fluorescent intensity of pStat92E in testes with indicated genotypes at different time points after eclosion. Mean ± SD is shown (n ≥ 45). Ordinary one-way ANOVA test was used. ∗∗∗∗p < 0.0001.

(K–O) Representative confocal images of Stat92E staining (red) in adult testes of control and nos > p115RNAi at different time points after eclosion. The levels of Stat92E gradually reduced over time in p115-depleted GSCs (white arrowheads). The hub is stained by FasIII in yellow. Stat92E channels are shown separately.

(P) Quantification of the average fluorescent intensity of Stat92E in testes with indicated genotypes ate different time points after eclosion. Mean ± SD is shown (n ≥ 55). Ordinary one-way ANOVA test was used. ∗∗∗∗p < 0.0001. Scale bars, 5 μm.