Figure 6.

Biochemical and genetics interactions between p115 and Stat92E

(A) Endogenous Stat92E (red) and transiently expressed p115-Flag (green, by Flag) partially co-localized in GSCs (white arrows). Asterisk marks the hub. Flag and Stat92E channels are shown separately.

(B) Transiently expressed Stat92E (red, by HA) and transiently expressed p115 (green, by Flag) partially co-localized in germline cells (white arrows). Flag and HA channels are shown separately.

(C) Physical interaction between p115 and Stat92E. Lysate from tub^ts flies was used as negative control.

(D–G) The phenotype of p115 knockdown (E) can be fully rescued by overexpression of Stat92E (F) compared with control (D). And overexpression of Stat92E has no obvious phenotype (G) compared with control (D). White arrowheads indicate GSCs. Vasa is stained for germline cells and FasIII is stained for the hub.

(H) Quantification of the number of GSCs per testis in testes with indicated genotypes. Mean ± SD is shown. n = 26 for nos > w^RNAi as control, n = 25 for nos > p115^RNAi, n = 27 for nos > Stat92E, p115^RNAi, and n = 23 for nos > Stat92E. Ordinary one-way ANOVA test was used, ns, not significant, ^∗∗∗∗p < 0.0001. DAPI is stained in blue for the nucleus. Scale bars, 5 μm in (A and B) and 10 μm (D–G).