Table 3.
Molecular weight of PHA synthesized by IF017-based strains of R. eutropha
Entry | Host | Plasmid | Composition [mol%] | Mw [× 105] |
PDI | Ref | ||
---|---|---|---|---|---|---|---|---|
3HB | 3HV | 2HB | ||||||
16 |
R. eutropha IF017 |
pBBR1MCS-2 | 89.3 ± 0.2 | 1.2 ± 0.0 | 9.5 ± 0.2 | 6.6 | 1.4 | This study |
17 | pBPP-phaCAR | 63.2 ± 0.8 | 1.8 ± 0.2 | 35.0 ± 0.8 | 3.3 | 1.4 | This study | |
E. coli JM109 |
pBSPRephaCARpct | 74.6a) | – | 25.6 ± 5.0 | 3.7 | 1.9 | Matsumoto et al. 2018 | |
59.4a) | – | 40.6 ± 0.3 | 3.9 | 1.9 |
Mw, weight-average molecular weight; PDI, poly dispersity index. a) Estimated from the 2HB composition