Fig. 7.
Overexpression of rat p53R211* mutant hinders TBK1-IRF3-STING trimeric complex formation and specifically reduces IL-17A expression in vivo. A Detection of p-TBK1, p-IRF3 and p-STING concentration in blood serum of the above treatment groups by ELISA assay. Blood serum was harvested from healthy controls, AAV-p53R211*- and AAV-EGFP-injected AIA rats. *p < 0.05, **p < 0.01, ***p < 0.0001 compared with AIA control group. B Representative immunofluorescence images (scale bar: 38.6 µm) demonstrate the synovial tissues from healthy controls, AAV-EGFP-injected and AAV-p53R211*-injected AIA rats (n = 5). Synovial tissues from five rats were sectioned and immune-stained with antibodies against p-TBK1, p-IRF3, p-STING and IL-17A prior to the treatment with anti-rabbit 2nd antibody conjugated with TRITC. The bar charts show the quantitative analysis of the immunofluorescent signals of the proteins by ImageJ software