FIGURE 5.

Complement activation of IgG1 and IgG3 is independent of complement regulators. Complement-dependent cytotoxicity (CDC) activity of IGHG1*03 (black), IGHG3*01 (long hinge, red), and IGHG3*04 (short hinge, pink) specific to trinitrophenyl (TNP) on Raji and Ramos B cells including CD46, CD55, and CD59 knockout cell lines at an Ag density of 0.5 mM (medium) TNBS. Negative controls are shown as dotted line for TNP unlabeled cells, and a dashed line represents TNPylated cells in the absence of Abs. Data represent mean ± SEM of n = 3.