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. 2022 Mar 16;149(6):dev200159. doi: 10.1242/dev.200159

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© 2022. Published by The Company of Biologists Ltd

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Fig. 6. — Rdx facilitates the downregulation of ptc expression in cells away from the compartment boundary. (A) In yw; ptc-GAL4(ptc^559.1)/+; UAS-TT/+ animals, the levels of destabilized GFP are reduced relative to that of RFP in ptc-expressing cells away from the compartment boundary; n=15. A1 shows GFP fluorescence. A2 shows RFP fluorescence. A3 shows a merged image (RFP, magenta; GFP, green). (B) In yw; ptc-GAL4(ptc^559.1)/+; UAS-TT, UAS-RNAi-rdx(v28798)/+ animals the level of destabilized GFP is not reduced relative to RFP in ptc-expressing cells away from the boundary; n=13. B1 shows GFP fluorescence. B2 shows RFP fluorescence. B3 shows a merged image (RFP, magenta; GFP, green). The bars labeled C-F in A and B correspond to the graphs below the figure. (C) Bar function of ImageJ used to graph the distribution of GFP (green line) and RFP (red line) from panels in A. (D) Bar function of ImageJ used to graph the distribution of GFP and RFP from panels in B. (E) Boxed cell away from the compartment boundary in panel A has reduced levels of destabilized GFP relative to RFP. (F) Boxed cell adjacent to the compartment boundary in panel A has elevated levels of destabilized GFP relative to RFP. Scale bar: 10 μm.