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. 2021 Aug 3;148(18):dev198671. doi: 10.1242/dev.198671

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© 2021. Published by The Company of Biologists Ltd

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Fig. 5. — Transcriptome analyses highlight key genes associated with the activation of dermal components in early growth-phase during feather cycling. (A) Diagram shows different dermal components in an early growth-phase feather follicle. (B) PCA analysis of four dermal components. (C) Strategy of finding marker genes in different dermal components. (D) DEG clustering analysis and the expression heat map. Clusters with marker genes are highlighted by dashed squares. (E) Marker gene expression levels in different dermal components. Data are mean±s.d. (F-I) Expression of FZD8 (F), TNC (G) and NCAM (H) in early growth-phase feather follicles revealed by RNAscope. (I) Merged images. Dotted lines in F-I outline the DP. cPP; central pulp; DP, dermal papilla; DS, dermal sheath; fe, feather epidermis; pPP, peripheral pulp.