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. 2023 Nov 17;14:7473. doi: 10.1038/s41467-023-43038-z

Fig. 1. In vivo biopanning for selection of VHH nanobodies that target the immune TME and assessment.

Fig. 1

a In vivo biopanning schema of VHH-nanobody phage display harvesting tumor and draining LN analyzed by NGS and viral titer then amplified CD45+ cells for a total of four rounds. b Each immune cell sub-population was sorted from immune-sensitive (Py117) and immune resistant (Py8119) tumors and LNs via ficoll gradient followed by magnetic beads separation for each round, then samples were sequenced to evaluate selection and enrichment. c NGS analysis pipeline for each round of biopanning. Reads were aligned to Alpaca IG reference, all regions of VHHs CDRs were assembled, followed by clonality assessment, diversity assessment, and clone tracking for enrichment. Llama and mouse illustrations were adapted from https://www.svgrepo.com/svg/162/llama, https://creazilla.com/nodes/7772730-mouse-clipart under creative commons license (CC0).