Fig. 7. DC-VHHs selection and differential binding of VHHs across immune cells and tissues.

a Pipeline to select VHHs that preferably bind to dendritic cells and then generate Venus-VHH fusions. b Purified fusion protein by ÄKTA pure detected by Coomassie blue staining, Venus fluorescence, and Immunoblot using α−His antibody (representative of 3 independent experiments). c Flowcytometry gating strategy to select cells sub-populations CD11c⁺, CD11b⁺, CD8⁺, and CD4⁺. d Detection of the selected Venus fusion DC-VHHs, BCII10-VHH, and DC2.1-VHH in the tumor, spleen, and LN of Py117 tumor-paired mice (representative experiment) from three independents experiments. Source data are provided as a Source Data file. Mouse illustration was adapted https://creazilla.com/nodes/19275-cartoon-grey-mouse-clipart under creative commons license (CC0).