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. 2023 Nov 17;13:351. doi: 10.1038/s41398-023-02648-3

Fig. 2. Animals cluster into three groups based on behavioral phenotypes.

Fig. 2

A Expression of significant PCs after behavioral analyses. Dots represent integrated z-scores from the four behavioral paradigms for each rat. Three groups of individuals (gray, blue, and purple dots) were separated by unsupervised k-meaning clustering (n = 9 naïve HBZ, n = 9 naïve LBZ, n = 14 stressed). B Representative contributions of each behavioral paradigm to the two PCA components. C Intergroup comparisons of the integrated behavioral z-score: naïve HBZ, naïve LBZ, and stressed animals (F2,29 = 317.6, p < 0.0001). D Naïve LBZ and stressed animals spent less time in the light zone of the LDB test (F2,29 = 31.32, p < 0.0001), and E presented reduced sociability (F2,29 = 62.71, p < 0.0001). Also, F both groups displayed a decreased discrimination index in the NOR test (F2,29 = 87.83, p < 0.0001). Data are shown as mean ± SEM. *p < 0.05; one-way ANOVA, followed by Tukey’s multiple comparison test.