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. 2023 Nov 17;14(11):751. doi: 10.1038/s41419-023-06271-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A PLD3 expression in stable PLD3-overexpression cells (231-PLD3) and control cells (231-Vector) was determined by the western blot. MTT (B) and colony-formation (C) analyzed the proliferation of the cells described in (A). Flow cytometry analyzed the apoptosis (D) and the cell cycle distribution (E) of the cells described in (A). F Tumor growth curves of the subcutaneous tumor made of the cells described in (A) at the indicated times and dissected tumors photographed at the harvest time, each group included 6 mice. G IHC staining analyzed the expression level of Ki-67 in breast cancer tissues with high or low PLD3 expression, the representative IHC image of each mouse has been selected for statistical analysis. *P < 0.05.