Table 3.
Polyadenylation activity for 17 variant hexamers as compared to AAUAAA, hexamers have been grouped according to polyadenylation activity.
| Sequence | Polyadenylation (% AAUAAA) | Classification Group |
|---|---|---|
| AAUAAA | 100 | Group 6 |
| AUUAAA | 77 ± 4.7 | Group 5 |
| AGUAAA | 29 ± 8.1 | Group 4 |
| CAUAAA | 18 ± 6.4 | Group 3 |
| UAUAAA | 17 ± 3.0 | Group 3 |
| ACUAAA | 11 ± 6.0 | Group 2 |
| GAUAAA | 11 ± 1.0 | Group 2 |
| AAUACA | 11 ± 2.3 | Group 2 |
| AAUAUA | 10 ± 2.3 | Group 2 |
| AAGAAA | 6.0 ± 1.0 | Group 1 |
| AACAAA | 4.0 ± 2.0 | Group 1 |
| AAAAAA | 4.6 ± 3.7 | Group 1 |
| AAUGAA | 4.3 ± 0.6 | Group 1 |
| AAUCAA | 4.0 ± 1.7 | Group 1 |
| AAUAAC | 3.7 ± 1.5 | Group 1 |
| AAUAGA | 3.3 ± 1.5 | Group 1 |
| AAUUAA | 2.3 ± 0.6 | Group 1 |
| AAUAAG | 1.7 ± 0.6 | Group 1 |
PS1/PM5 can be awarded at a moderate level when a variant creates a hexamer that falls into the same (or lower) group as the previously classified variant. Sheets et al.