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. 2023 Sep 2;32(23):3263–3275. doi: 10.1093/hmg/ddad140

Figure 4.

Figure 4

Mutating the C-terminal dilysine motif does not impact normal Nucleolin function. (a) Representative micrographs of NSC-34 cells transfected with LifeAct Ruby (red) and eGFP-tagged Nucleolin. Nuclei were visualized with DAPI (blue). (b) Representative Western blot of HEK 293TT cells transfected with HA-NCLWT or HA-NCLΔKK shows equal cell-surface expression. (c) DNA gels showing RT-PCR from inputs or RIPs in untransfected control HEK 293TT compared to cells transfected with HA-NCLWT or HA-NCLΔKK.