Figure 5.
Nucleolin mediates binding of α-COP to some mRNA. (a) Venn diagram showing overlap of Nucleolin-associated mRNA in Hela cells with α-COP-associated mRNA in NSC-34 cells and proposed model of Nucleolin acting as an adapter between mRNA and α-COP. (b) Representative Western blot of whole cell lysates from stable shGFP and shNCL HEK 293TT lines shows decrease in Nucleolin at the protein level. Immunoprecipitation of endogenous α-COP with a rabbit polyclonal antibody (GTX) was approximately equal in both lines. (c) Quantitative RT-PCR of α-COP RIPs normalized to GAPDH. (d) Quantitative RT-PCR from RIPs comparing α-COPWT to α-COPE269V. A representative Western blot confirms successful expression and immunoprecipitation of the Myc-tagged α-COP.