Skip to main content
. 2023 Sep 2;32(23):3263–3275. doi: 10.1093/hmg/ddad140

Figure 6.

Figure 6

Loss of Nucleolin binding reduces the ability of α-COP to promote neurite outgrowth. (a) Representative micrographs of β-III tubulin labeled NSC-34 cells from control (pBabe + pCI-neo) Copa knockdown (shCOPA) and Myc-FLAG-α-COP rescue lines (WT or EV) after 5 days of differentiation. (b) Normalized neurite length quantification from 3 biological replicates measured by a blinded observer shows significant decrease in neurite length following Copa knockdown (p < 0.001). Stable expression of α-COPWT significantly restored neurite length (p < 0001). Stable expression of α-COPE269V rescued neurite length to some degree, but neurites were significantly shorter compared to α-COPWT cultures (p = 0.01). Error bars represent standard error of the mean. (c) Representative Western blot of whole cell lysates confirming expression of Myc-FLAG-α-COPWT and Myc-FLAG-α-COPE269V. Representative Western blot of whole cell lysates with antibody against endogenous α-COP confirms reduced α-COP protein in shRNA lines and restoration to normal levels by stable expression of Myc-FLAG-α-COPWT and Myc-FLAG-α-COPE269V.