Table 1.
Activity screening of whole-cell lysates of E. coli NEB5α expressing the individual enzymes for the two-step conversion of ferulic acid into coniferyl alcohol via coniferyl aldehyde
| Enzyme | Substrate | Product yield | |
|---|---|---|---|
| Coniferyl aldehyde (%) | Coniferyl alcohol (%) | ||
| NiCAR | Ferulic acid | 4.6 | 0.02 |
| SrCAR | Ferulic acid | 28.2 | 0.26 |
| PsCAD | Coniferyl aldehyde | - | 14.5 |
| MsCAD2 | Coniferyl aldehyde | - | 19.3 |
| CgAKR1 | Coniferyl aldehyde | - | 4.5 |
| NiCAR + PsCAD | Ferulic acid | 3.3 | 17.9 |
| NiCAR + MsCAD2 | Ferulic acid | 1.1 | 33.6 |
| NiCAR + CgAKR1 | Ferulic acid | 2.3 | 13.3 |
| SrCAR + PsCAD | Ferulic acid | 12.1 | 26.0 |
| SrCAR + MsCAD2 | Ferulic acid | 6.3 | 50.3 |
| SrCAR + CgAKR1 | Ferulic acid | 20.2 | 8.4 |