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. 2023 Nov 18;22:184. doi: 10.1186/s12943-023-01871-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — ELF5 inhibits RCC progression by upregulating WDTC1. (A) RT-qPCR result of WDTC1 expression in HK-2, 769-P, and A498 cells. (B) Colony formation assay was employed for detecting cell proliferative capability when WDTC1 was overexpressed. (C-E) Transwell assay was carried out for testing cell migratory and invasive capabilities. (F-G) Tube formation assay was employed for testing angiogenesis of HUVECs. (H-I) RT-qPCR and western blot outcomes of VEGFA level.(J) Tumor weight and the tumor growth curve were measured in WDTC1 overexpression cell and its control group (K-P) Cell proliferation (k), migration (l), invasion (m), angiogenesis (n), and VEGFA expression (o-p) were detected by colony formation assay (k), transwell assay (l-m), tube formation assay (n), RT-qPCR (o), and western blot (p) in cells of the pcDNA3.1 group, the pcDNA3.1-ELF5 group, the pcDNA3.1-ELF5 + sh-NC group, and the pcDNA3.1-ELF5 + sh-WDTC1 group. Two-tailed Student’s t test was performed. The error bars represent SD. *p < 0.05, **p < 0.01, and ***p < 0.001