TABLE 1.
Sequence type distribution
| Sequence typea | % of type found in the following libraryb:
|
Putative division | Database match (≥95% identity) | |||||
|---|---|---|---|---|---|---|---|---|
| WCHA | WsCH | WCHB | WCHD | WSA | WFeA | |||
| WCHA2-13 | <1 | Acidobacteria | ||||||
| WCHB1-08, -18 | 1 | Actinobacteria | ||||||
| WCHB1-81 | <1 | 1 | Actinobacteria | |||||
| WFeA1-02 | 3 | Actinobacteria | ||||||
| WCHA1-01, -14, -2-47 | <1 | Cytophagales | ||||||
| WCHB1-29, -32, -53 | 1 | Cytophagales | ||||||
| WCHB1-69 | 2 | 1 | Cytophagales | |||||
| WsCH39 | 1 | Cytophagales | 95% Chryseobacterium indologenes (M58773) | |||||
| WCHA1-69 | 3 | 1 | 1 | Green nonsulfur bacteria | ||||
| WCHB1-05 | 5 | 1 | Green nonsulfur bacteria | |||||
| WCHB1-31 | 1 | 3 | 2 | Green nonsulfur bacteria | ||||
| WCHB1-43, -44, -50, -57, -80 | 1 | Green nonsulfur bacteria | ||||||
| WCHB1-62 | 1 | 1 | 2 | Green nonsulfur bacteria | ||||
| WCHB1-63 | 1 | Green nonsulfur bacteria | 98% Anaerobic digestor clone HB28 (U81745) | |||||
| WCHA1-94 | <1 | Green sulfur bacteria | ||||||
| WCHA1-17, -45, -53 | <1 | Low-G+C gram positive | ||||||
| WCHB1-20 | 1 | Low-G+C gram positive | ||||||
| WCHB1-21 | <1 | 6 | 2 | Low-G+C gram positive | ||||
| WCHB1-49, -54, -84 | 1 | Low-G+C gram positive | ||||||
| WCHB1-71 | 1 | 1 | Low-G+C gram positive | |||||
| WCHB1-77 | 1 | Low-G+C gram positive | 95% Desulfotomaculum orientis (Y11570) | |||||
| WCHB1-82 | 1 | Low-G+C gram positive | ||||||
| WCHB1-89 | 1 | Low-G+C gram positive | 95% Anaerobic digestor clone HB76 (U81754) | |||||
| WFeA1-16 | 4 | Low-G+C gram positive | 99% Exiguobacterium aurantiacum (X70316) | |||||
| WsCH1, 5, 8 | 1 | Low-G+C gram positive | ||||||
| WCHA1-79 | <1 | 2 | OP10 | |||||
| WsCH12 | 1 | OP10 | ||||||
| WCHA1-09 | 2 | 1 | OP11 | |||||
| WCHA1-11, -16, -20, -24; 2-01, -26 | <1 | OP11 | ||||||
| WCHB1-07 | 2 | OP11 | ||||||
| WCHB1-11 | <1 | 1 | OP11 | |||||
| WCHB1-26 | 13 | 18 | 2 | 6 | OP11 | |||
| WCHB1-56 | <1 | 2 | 1 | OP11 | ||||
| WCHB1-58 | 1 | OP11 | ||||||
| WCHB1-64 | <1 | 3 | OP11 | |||||
| WCHB1-02 | 1 | OP5 | ||||||
| WCHB1-03 | 3 | 2 | 8 | 3 | 8 | OP5 | ||
| WCHA1-39 | <1 | OP8 | ||||||
| WCHA1-83 | 1 | OP8 | ||||||
| WFeA1-35 | 1 | OP8 | ||||||
| WFeA1-59 | 1 | 7 | OP8 | |||||
| WCHA1-65 | <1 | Proteobacteria (α) | ||||||
| WCHB1-55 | 1 | Proteobacteria (α) | ||||||
| WCHB1-87 | 1 | Proteobacteria (α) | ||||||
| WCHA1-52 | <1 | Proteobacteria (β) | 98% Azoarcus sp. strain BS1-14 (AF011348) | |||||
| WCHA1-62 | 1 | 6 | 1 | Proteobacteria (β) | 98% Denitrifying Fe-oxidizing bacterium (U51101) | |||
| WFeA1-06c | 17 | Proteobacteria (β) | 97% Duganella zoogloeoides (X74914) | |||||
| WsCH66 | 1 | Proteobacteria (β) | ||||||
| WCHA1-76 | <1 | Proteobacteria (γ) | 95% Legionella birminghamensis (Z49717) | |||||
| WCHA1-85 | <1 | Proteobacteria (γ) | ||||||
| WCHA1-07 | <1 | Proteobacteria (δ) | 95% Syntrophus gentianae (X85132) | |||||
| WCHB1-12 | 7 | 16 | 7 | 4 | Proteobacteria (δ) | 95% Syntrophus gentianae (X85132) | ||
| WCHB1-27, -67 | 1 | Proteobacteria (δ) | ||||||
| WFeA1-10 | 4 | Proteobacteria (δ) | ||||||
| WsCH54 | 2 | Proteobacteria (δ) | ||||||
| WCHB1-30 | 4 | Spirochetes | ||||||
| WCHB1-40, -91 | 1 | Spirochetes | ||||||
| WCHA1-89 | <1 | Termite group I | ||||||
| WCHA1-33 | <1 | Verrucomicrobia | ||||||
| WCHA1-80 | 2 | 1 | Verrucomicrobia | |||||
| WCHB1-25 | 2 | Verrucomicrobia | ||||||
| WCHB1-41 | 1 | 1 | Verrucomicrobia | |||||
| WCHD3-88 | 1 | 1 | Verrucomicrobia | |||||
| WCHA1-48 | <1 | WS1 | ||||||
| WCHA1-78 | 2 | 2 | 1 | WS1 | ||||
| WCHA1-37 | <1 | WS2 | ||||||
| WCHA1-56 | <1 | WS3 | ||||||
| WsCH31 | 3 | WS4 | ||||||
| WCHB1-60 | 1 | WS5 | ||||||
| WCHA | WsCH | WCHB | WCHD | WSA | WFeA | |||
| WCHB1-01 | 13 | WS6 | ||||||
| WCHB1-06 | 7 | WS6 | ||||||
| WCHB1-15 | 4 | WS6 | ||||||
| WCHA1-38 | <1 | Crenarchaeota | 96% Marine clone C20 (U71114) | |||||
| WCHA1-57, 2-08 | <1 | Euryarchaeota | ||||||
| WCHD3-02 | 8 | Euryarchaeota | ||||||
| WCHD3-03 | 8 | 3 | 81 | 9 | Euryarchaeota | 97% Methanosaeta concilii (X16932) | ||
| WCHD3-07 | 3 | Euryarchaeota | 97% Methanospirillum sp. (L48407) | |||||
| WCHD3-16, -33 | 1 | Euryarchaeota | ||||||
| WCHD3-30 | <1 | 1 | Euryarchaeota | |||||
| WCHD3-34 | 2 | Euryarchaeota | ||||||
a
Groups of two or more highly related sequences (≥98% identical) are considered to belong to the same sequence type. Sequences listed on the same line represent different types belonging to the same division and detected only once within a library.
b
Based on direct sequence comparisons or inferred from RFLP patterns. Percentages do not add up to 100% because not all unique RFLPs were sequenced.
c
Potential contaminant (42).