Fig. 1.

HDAC6- LINC00152- hsa-miR-499a-5p network in multiple myeloma. (A) Workflow for the identification of potential regulatory LINC00152 miRNA-HDAC6 networks. (B) Overlapping miRNA was identified using relevant public databases (GSE125363, miRDB, miRWalk and prediction of miRNAs targeting LINC00152 from miRcode (highconsfamilies dataset)). (C) Volcano plot for screening the lncRNAs differentially expressed in multiple myelomas obtained from GEO dataset GSE47552. (D) LINC00152 acted as a sponge for hsa-miR-499a-5p in MM cells. Schematic representation of the binding sites between has-miR-499a-5p and HDAC6 3’UTR. (E) Relative mRNA expression levels of LINC00461 and hsa-miR-499a-5p in MM cell lines (OPM-2 and U266) treated with either DMSO or 2 µM HDAC6 inhibitor (ACY-1215). Results represent data from three separate experiments. Data are presented as mean ± standard deviation (SD). (p < 0.05, unpaired Student’s t-test). (F) HDAC class IIb (HDAC6 and HDAC10) and HDAC class IIa (HDAC4, HDAC5, HDAC7 and HDAC9) showed significant prognostic ability in multiple myeloma retrieved from MMRF-COMMpass database